Research Paper
Year: 2022 | Month: July-September | Volume: 6 | Issue: 3 | Pages: 116-138
DOI: https://doi.org/10.52403/gijash.20220715
Studies on Purification and Characterization of Sweet Potato (Ipomoea Batatas) Cultivated in the Northern Part of Bangladesh
Most.Lutfunnahar1, Manoshi Sana2, Samiron Sana3, Md Shajedul Haque4, Badhan Rani Dey5, Hasi Rani Saha6, Bidhan Chandra Sarkar7, Niranjan Kumar Sana8
1Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh
2Dhaka Medical College, Dhaka
3Pharmacy Discipline, Khulna University, Khulna-9208, Bangladesh
4Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh
5Department of Biochemistry and Molecular Biology, Primeasia University, 12 Kemal Ataturk Avenue, Dhaka-1213, Bangladesh
6Department of Biochemistry and Molecular Biology, Primeasia University, 12 Kemal Ataturk Avenue, Dhaka-1213, Bangladesh
7Department of Biochemistry and Molecular Biology, Primeasia University, 12 Kemal Ataturk Avenue, Dhaka-1213, Bangladesh
8Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh
Corresponding Author: Dr. Bidhan Chandra Sarkar
ABSTRACT
Enzyme activity was also determined from the Red sweet potato and white sweet potato. The activities of Amylase, Cellulase, Invertase, & protease were 0.093±0.01, 0.05±0.02, 0.062± 0.015, 0.043± 0.02, for Red while 0.088± 0.01, 0.073 ±0.02, 0.072 ±0.015 & 0.050± 0.02 for White sweet potato. Amylase was partially purified from the extract of sweet potato using DEAE cellulose column chromatography. On the purity checking and molecular weight determination on SDS-PAGE. The molecular weight was determined by SDS-PAGE to be 64±1 kDa. The partially purified amylase from sweet potato is β-type is confirmed by HgCl2.The optimum pH & temperatures of partially purified β-amylase were 7.5 and 55◦c respectively. Amylolytic activity of this enzyme is 105% and 55% for amylopectin and Amylase. This enzyme does not affect maltose and maltotetraose. Metallic ions like Fe3+, Mn2+, Mg2+, and EDTA increased amylase activity while other ions like Cu+2, Hg+2, Pb+2, and urea reduced the amylase activity. The study indicates the importance of β-amylase as a starch degrading enzyme. This enzyme showed less toxicity towards brine shrimp nauplii with the Lc50 value of 1970.288µg/ml. This enzyme did not show any antibacterial activity against Staphylococcus aureus, Escherichia coli, Salmonella enterica, Bacillus subtilis.
Keywords: Sweet potato, Ipomoea Batatas, Starch, Amylase, Protease.
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